产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
SK-N-SH , mouse hippocampus , rat hippocampus , rat spinal cord
预测种属反应
Bovine, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.92 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Synthetic peptide encompassing a sequence within the Intracellular domain of mouse KCC2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886837
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
solute carrier family 12, member 5 , KCC2 , mKIAA1176
细胞定位
Cell projection , dendrite , Cell membrane; Multi-pass membrane protein
功能与背景
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
数据库
研究领域
產品图片
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GTX133130 IHC-P Image
KCC2 antibody detects KCC2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: KCC2 protein stained by KCC2 antibody (GTX133130) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133130 WB Image
Human brain (5 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133130 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133130 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133130 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133130 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184
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GTX133130 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184
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GTX133130 ICC/IF Image
KCC2 antibody detects KCC2 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KCC2 protein stained by KCC2 antibody (GTX133130) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX133130 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:500.
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GTX133130 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody (GTX133130) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29028184
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文献引用
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