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KDM6A抗体 [HL1731]

Recombinant antibody KO/KD Orthogonal
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379
Anti-KDM6A antibody [HL1731] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637379

目录号 GTX637379

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1731

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

154 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lysine demethylase 6A , KABUK2 , UTX , bA386N14.2

细胞定位

Nucleus

功能与背景

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]

数据库

研究领域

產品图片

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

KO/KD

Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KDM6A antibody [HL1731] used in Western Blot (WB). GTX637379

GTX637379 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [HL1731] (GTX637379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat tissue.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in IHC (Paraffin sections) (IHC-P). GTX637379

GTX637379 IHC-P Image

KDM6A antibody [HL1731] detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KDM6A antibody [HL1731] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637379

GTX637379 ICC/IF Image

KDM6A antibody [HL1731] detects KDM6A protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KDM6A stained by KDM6A antibody [HL1731] (GTX637379) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

文献引用

Application Reference

Zhao Y et al. Cell Stress Chaperones 2023; 28 (4) : 375-384 ROS inhibition increases KDM6A-mediated NOX2 transcription and promotes macrophages oxidative stress and M1 polarization.
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产品包装 报价 (¥)
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¥ 1700
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