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KLF5抗体 [HL2269]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321
Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

目录号 GTX638321

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2269

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

51 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human KLF5.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

细胞定位

Nucleus

功能与背景

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

数据库

研究领域

產品图片

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

KO/KD

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321

GTX638321 IHC-P Image

KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in IHC (Paraffin sections) (IHC-P). GTX638321

GTX638321 IHC-P Image

KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
KLF5 stained by KLF5 antibody [HL2269] (GTX638321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KLF5 antibody [HL2269] used in Western Blot (WB). GTX638321

GTX638321 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (GTX638321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
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¥ 1700
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