产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant antigen containing the carboxy terminal portion of human Ku80 protein
纯化方式
Protein G purified
偶联
Unconjugated
RRID
AB_372053
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV
细胞定位
Nucleus , Nucleolus
功能与背景
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/templates/default/js/lightbox/images/loading.gif "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
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GTX70276 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [149.8] (GTX70276) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70276 WB Image
Detection of human Ku80 protein in HeLa whole cell lysate using GeneTex Ku80 149.8 monoclonal antibody GTX70276.
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GTX70276 WB Image
Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70276 diluted at 1:10000
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GTX70276 WB Image
Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX70276 diluted at 1:10000
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GTX70276 ICC/IF Image
Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku80 protein stained by Ku80 antibody [149.8] (GTX70276) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX70276 ICC/IF Image
The data was published in the journal J Virol in 2017.PMID: 28855246
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GTX70276 WB Image
The data was published in the journal J Virol in 2017.PMID: 28855246
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文献引用
产品评论
目前没有使用 Ku80 antibody [149.8] (GTX70276) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/small/GTX70276/GTX70276_40980_20211105_WB_w_23061221_172.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/small/GTX70276/Ku80-antibody-149.8-GTX70276-WB-1_w_23061221_188.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/small/GTX70276/Ku80-antibody-149.8-GTX70276-WB-3_w_23061221_505.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/small/GTX70276/Ku80-antibody-149.8-GTX70276-WB-2_w_23061221_115.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276](/upload/website/prouct_img/small/GTX70276/GTX70276_40980_IFA_w_23061221_685.webp "Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276")
![Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276](/upload/website/prouct_img/small/GTX70276/GTX70276_20240411_citeab_ICCIF_510_24041501_813.webp "Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/small/GTX70276/GTX70276_20240411_citeab_WB_509_24041501_718.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/normal/GTX70276/GTX70276_40980_20211105_WB_w_23061221_172.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/normal/GTX70276/Ku80-antibody-149.8-GTX70276-WB-1_w_23061221_188.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/normal/GTX70276/Ku80-antibody-149.8-GTX70276-WB-3_w_23061221_505.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/normal/GTX70276/Ku80-antibody-149.8-GTX70276-WB-2_w_23061221_115.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
![Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276](/upload/website/prouct_img/normal/GTX70276/GTX70276_40980_IFA_w_23061221_685.webp "Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276")
![Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276](/upload/website/prouct_img/normal/GTX70276/GTX70276_20240411_citeab_ICCIF_510_24041501_813.webp "Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276")
![Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276](/upload/website/prouct_img/normal/GTX70276/GTX70276_20240411_citeab_WB_509_24041501_718.webp "Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276")
