产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1 - 2 μg/mL |
| 10 μg/mL |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900)
产品说明
LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2.The immunogen is located within the last 50 amino acids of LAMP-2.
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
RRID
AB_1952757
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110
细胞定位
Cell membrane,Endosome membrane,Lysosome membrane
功能与背景
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing DanonOs disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).
数据库
研究领域
產品图片
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GTX30881 WB Image
WB analysis of HepG2 cell lysate using GTX30881 LAMP2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX30881 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX30881 LAMP2 antibody.
Working concentration : 10 μg/ml
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GTX30881 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX30881 LAMP2 antibody.
Dilution : 5 μg/ml
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文献引用
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