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LC3A抗体 [166AT1234]

Orthogonal Overexpression
Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

目录号 GTX17380

文献引用 16

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 166AT1234

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IHC

种属反应

 Human, Mouse, Rat
产品包装
200 μl (¥5600)
查看所有 LC3A 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
ICC/IF 1:25-200
IHC-P Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

This LC3A antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

纯化方式

Protein G purified, followed by dialysis against PBS.

偶联

Unconjugated

RRID

AB_2887560

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

Orthogonal

ICC/IF analysis of U251 cells untreatd (left) or treated with Chloroquine (50 μM,16h) (right) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : DAPI
Dilution : 1:25

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

WB analysis of various samples using GTX17380 LC3A antibody [166AT1234].
Lane 1: human brain lysates
Lane 2: mouse brain lysates
Lane 3: rat brain lysates
Dilution : 1:2000

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Overexpression

WB analysis of multiple cell lysate using GTX17380 LC3A antibody [166AT1234].
Lane 1: Y79 (soluble fraction of cell extract)
Lane 2: 293 transfected with human LC3A (whole cell extract)
Dilution : 1:1000

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

ICC/IF analysis of U251 cells treated with Chloroquine (50 μM,16h) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : cell nuclear
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.2%, 30 min)
Dilution : 1:200

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

Orthogonal

The data was published in the Cancer Med in 2020. PMID: 32324343

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

The data was published in the Cancer Med in 2020. PMID: 32324343

文献引用

Application Reference

Lopez-Toledo G et al. Front Aging Neurosci 2022; 14 : 921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
实验应用 : WB
种属反应 : Human
Jing Z et al. Cancer Med 2020; 9 (12) : 4324-4338 SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR-23b-3p via sponging EGR1 in hepatocellular carcinoma.
Schultz ML et al. Nat Commun 2018; 9 (1) : 3671 Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.
Shih CH et al. Part Fibre Toxicol 2018; 15 (1) : 44 Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.
实验应用 : IHC
种属反应 : Rat

Clone Reference

He Z et al. Mol Med Rep 2015; 11 (6):914416 * induces autophagic cell death in prostate cancer cells in vitro.
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Application Reference
Lopez-Toledo G et al. Front Aging Neurosci 2022; 14:921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
实验应用 : WB
种属反应 : Human
Jing Z et al. Cancer Med 2020; 9 (12):4324-4338 SNGH16 regulates cell autophagy to promote Sorafenib Resistance through suppressing miR-23b-3p via sponging EGR1 in hepatocellular carcinoma.
Schultz ML et al. Nat Commun 2018; 9 (1):3671 Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.
Shih CH et al. Part Fibre Toxicol 2018; 15 (1):44 Chronic pulmonary exposure to traffic-related fine particulate matter causes brain impairment in adult rats.
实验应用 : IHC
种属反应 : Rat
Clone Reference
Rabionet M et al. Hum Mol Genet. 2015; 24 (17):4792-808 Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
Vlahava VM et al. Cell Signal 2015; 27 (6):1253-63 CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Hsin IL et al. Mol Pharm 2015; 12 (5):1534-43 gmi, an immunomodulatory protein from ganoderma microsporum, potentiates *-induced apoptosis via autophagy in lung cancer cells.
种属反应 : Human
He Z et al. Mol Med Rep 2015; 11 (6):914416 * induces autophagic cell death in prostate cancer cells in vitro.
Islam MA et al. Biomaterials 2014; 35 (5):1584-96 The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice.
种属反应 : Mouse
Zhang Y et al. Blood 2012; 119 (12):2895-905 Induction of autophagy is essential for monocyte-macrophage differentiation.
实验应用 : WB
种属反应 : Human
Zhou D et al. J Infect Dis 2012; 205 (8):1258-67 production of * α by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy.
实验应用 : WB
种属反应 : Human
Xing S et al. Autophagy 2012; 8 (1):63-76 Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
实验应用 : WB
种属反应 : Human
Song D et al. J Biol Chem 2011; 286 (18):15797-805 Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis.
实验应用 : WB
种属反应 : Mouse
Tuffy LP et al. Mol Cell Biol 2010; 30 (23):5484-501 Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
实验应用 : WB
种属反应 : Mouse
Rouschop KM et al. J Clin Invest 2010; 120 (1):127-41 The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
实验应用 : WB
种属反应 : Human
Byun JY et al. Carcinogenesis 2009; 30 (11):1880-8 The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
实验应用 : WB
种属反应 : Rat
SDS
PBS.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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