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LC3B抗体 [GT1187]

Recombinant antibody KO/KD Orthogonal
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949
Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949
Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

目录号 GTX00949

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 GT1187

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥5000)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

Observed MW

14, 16 kDa. The observed M.W. is based on the following publication. PMID: 31576176

产品属性

形式

Liquid

存储溶液

PBS, 50% Glycerol

保存剂

0.02% Sodium azide

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A synthetic peptide of human LC3B.

纯化方式


Purified by affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B , MAP1LC3Ba , APG8b

细胞定位

Cytoplasm,Cytoskeleton,Endomembrane system,Cytoplasmic vesicle,Autophagosome membrane,Autophagosome

功能与背景

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

Orthogonal

ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

Orthogonal

ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

Orthogonal

IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949

GTX00949 IHC-P Image

IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949

GTX00949 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

KO/KD Orthogonal

WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg

Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949

GTX00949 WB Image

KO/KD Orthogonal

WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg

文献引用

Application Reference

Q Cai et al. Nat Commun 2024; 15 (1) : 3563 LSD1 inhibition circumvents glucocorticoid-induced muscle wasting of male mice
实验应用 : WB
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5000
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