产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
14, 16 kDa. The observed M.W. is based on the following publication. PMID: 31576176
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthetic peptide of human LC3B.
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B , MAP1LC3Ba , APG8b
细胞定位
Cytoplasm,Cytoskeleton,Endomembrane system,Cytoplasmic vesicle,Autophagosome membrane,Autophagosome
功能与背景
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/templates/default/js/lightbox/images/loading.gif "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
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GTX00949 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00949 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
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GTX00949 ICC/IF Image
ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
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GTX00949 IHC-P Image
IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
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GTX00949 IHC-P Image
IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
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GTX00949 IHC-P Image
IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
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GTX00949 ICC/IF Image
ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
|
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GTX00949 WB Image
WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg
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GTX00949 WB Image
WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg
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文献引用
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产品评论
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![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_40000000144_20200306_WB_treatment_Thapsigargin_w_23053121_647.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_ICC-IF_43_w_23053121_449.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_ICC-IF_44_w_23053121_674.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_IHC-P_41_w_23053121_323.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_IHC-P_39_w_23053121_454.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_IHC-P_40_w_23053121_926.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_ICC-IF_42_w_23053121_487.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_WB_2_w_23053121_126.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/small/GTX00949/GTX00949_20200327_WB_1_w_23053121_206.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_40000000144_20200306_WB_treatment_Thapsigargin_w_23053121_647.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_ICC-IF_43_w_23053121_449.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_ICC-IF_44_w_23053121_674.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_IHC-P_41_w_23053121_323.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_IHC-P_39_w_23053121_454.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_IHC-P_40_w_23053121_926.webp "Anti-LC3B antibody [GT1187] used in IHC (Paraffin sections) (IHC-P). GTX00949")
![Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_ICC-IF_42_w_23053121_487.webp "Anti-LC3B antibody [GT1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00949")
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_WB_2_w_23053121_126.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
![Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949](/upload/website/prouct_img/normal/GTX00949/GTX00949_20200327_WB_1_w_23053121_206.webp "Anti-LC3B antibody [GT1187] used in Western Blot (WB). GTX00949")
