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LC3B抗体 [GT3612]

Orthogonal Overexpression
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632501

目录号 GTX632501

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT3612

同种型

 IgG2a

实验应用

 WB, ICC/IF

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

阳性对照

LC3B-transfected 293T , HepG2 (3 μM Thapsigargin treated for 12hr) , HeLa (50 μM chloroquine treated for 24hr)

预测种属反应

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_2888317

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

细胞定位

Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor

功能与背景

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632501

GTX632501 ICC/IF Image

LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody [GT3612] (GTX632501) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

文献引用

Application Reference

Zhang M et al. J Pharmacol Sci 2021; 147 (3) : 234-244 Liraglutide ameliorates diabetes-associated cognitive dysfunction via rescuing autophagic flux.
实验应用 : ICC/IF
Wan C et al. Cell Death Dis 2019; 10 (6) : 392 Silver nanoparticles selectively induce human oncogenic 帠-herpesvirus-related cancer cell death through reactivating viral lytic replication.
实验应用 : WB
种属反应 : Human
Tsai YG et al. Sci Rep 2018; 8 (1) : 12973 Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium.
实验应用 : WB
种属反应 : Human
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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