产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide of human LC3B.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a
细胞定位
Cytoplasm, cytoskeleton
功能与背景
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX639686 IHC-P Image
LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639686 IHC-P Image
LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639686 IHC-P Image
LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX639686 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639686 WB Image
Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639686 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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