产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , HeLa
预测种属反应
Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950805
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
LIM domain binding 1 , CLIM-2 , CLIM2 , LDB-1 , NLI
细胞定位
Nucleus
功能与背景
Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
数据库
研究领域
產品图片
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/templates/default/js/lightbox/images/loading.gif "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
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GTX104531 WB Image
Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX104531 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104531 WB Image
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104531 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX104531 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GTX104531) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104531 ICC/IF Image
LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDB1 protein stained by LDB1 antibody [N2C3] (GTX104531) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX104531 IHC-P Image
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104531 IP Image
LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 μg 293T whole cell lysate/extract
A. 20 μg 293T whole cell lysate/extract
B. Control with 2.5 μg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 μg of LDB1 antibody (GTX104531)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (GTX104531) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX104531 IHC-P Image
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献引用
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产品评论
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![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_41766_20181109_WB_KO_watermark_w_23060120_703.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_41766_20210514_WB_w_23060120_540.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_40079_WB_M_brain_w_23060120_914.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_IHC_w_23060120_645.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_40709_20150513_IFA_w_23060120_173.webp "Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_41073_IHC_M_w_23060120_717.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_40079_IP_w_23060120_839.webp "Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/small/GTX104531/GTX104531_41073_IHC_R_w_23060120_549.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_41766_20181109_WB_KO_watermark_w_23060120_703.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_41766_20210514_WB_w_23060120_540.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_40079_WB_M_brain_w_23060120_914.webp "Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_IHC_w_23060120_645.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_40709_20150513_IFA_w_23060120_173.webp "Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_41073_IHC_M_w_23060120_717.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
![Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_40079_IP_w_23060120_839.webp "Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531")
![Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531](/upload/website/prouct_img/normal/GTX104531/GTX104531_41073_IHC_R_w_23060120_549.webp "Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531")
