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LDH-B抗体

KO/KD Orthogonal
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747
Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747

目录号 GTX101747

文献引用 4
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

37 kDa. ( Note )

阳性对照

mouse brain , rat brain

预测种属反应

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human LDH-B. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037332

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lactate dehydrogenase B , HEL-S-281 , LDH-B , LDH-H , LDHBD , TRG-5

细胞定位

Cytoplasm

功能与背景

This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

KO/KD

Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747

GTX101747 IHC-P Image

LDH-B antibody detects LDH-B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
LDH-B stained by LDH-B antibody (GTX101747) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747

GTX101747 IHC-P Image

LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
LDH-B antibody (GTX101747) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747

GTX101747 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B(GTX101747) antibody at 1:50 dilution.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Orthogonal

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

文献引用

Application Reference

Goto T et al. Exp Hematol 2019; (Epub) ATP produced by anaerobic glycolysis is essential for enucleation of human erythroblasts.
实验应用 : WB
种属反应 : Human
Chen CN et al. Am J Physiol Regul Integr Comp Physiol 2017; : ajpregu.00337.2016 Diet-induced obesity accelerates blood lactate accumulation of rats in response to incremental exercise to maximum.
实验应用 : WB
种属反应 : Rat
Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
实验应用 : WB
种属反应 : Human
Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
实验应用 : WB
种属反应 : Human
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产品评论

LDH-B antibody 目录号 GTX101747

Rating
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实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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