LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (2 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 3 min

LGR5 antibody detects LGR5 protein at cell membrane by immunofluorescent analysis.
Sample: COLO 205 (high expression) and RMS13 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).