Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with LIF antibody [N1C3] (GTX101021) diluted at 1:500 and competitor's antibody (sc-1336) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.