产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Recombinant fragment of mouse LRP4
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
low density lipoprotein receptor-related protein 4 , 6430526J12Rik , D230026E03 , Megf7 , mdig
细胞定位
Cell membrane
功能与背景
Enables protein homodimerization activity and receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of presynaptic membrane organization; positive regulation of skeletal muscle acetylcholine-gated channel clustering; and synapse organization. Acts upstream of or within several processes, including animal organ development; embryonic digit morphogenesis; and regionalization. Located in neuromuscular junction and plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; eye; and genitourinary system. Used to study sclerosteosis 2. Human ortholog(s) of this gene implicated in Cenani-Lenz syndactyly syndrome; congenital myasthenic syndrome 17; and sclerosteosis 2. Orthologous to human LRP4 (LDL receptor related protein 4). [provided by Alliance of Genome Resources, Apr 2022]
数据库
研究领域
產品图片
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GTX637988 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637988 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637988 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637988 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (GTX637988) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637988 ICC/IF Image
LRP4 antibody [HL2080] detects LRP4 protein at cell membrane and nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LRP4 stained by LRP4 antibody [HL2080] (GTX637988) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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