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LYN抗体

KO/KD Orthogonal
Anti-LYN antibody used in Western Blot (WB). GTX101222
Anti-LYN antibody used in Western Blot (WB). GTX101222
Anti-LYN antibody used in Western Blot (WB). GTX101222
Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101222
Anti-LYN antibody used in Western Blot (WB). GTX101222
Anti-LYN antibody used in Immunoprecipitation (IP). GTX101222
Anti-LYN antibody used in IHC (Paraffin sections) (IHC-P). GTX101222

目录号 GTX101222

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 LYN 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

阳性对照

Raji , K562 , THP-1 , HeLa

预测种属反应

Rat, Sheep, Bovine, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human LYN. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10720516

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn

数据库

研究领域

產品图片

Anti-LYN antibody used in Western Blot (WB). GTX101222

GTX101222 WB Image

Orthogonal

Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX101222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LYN antibody used in Western Blot (WB). GTX101222

GTX101222 WB Image

KO/KD Orthogonal

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX101222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed effect is based on the publication: PMID: 17599831

Anti-LYN antibody used in Western Blot (WB). GTX101222

GTX101222 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX101222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LYN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101222

GTX101222 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX101222) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-LYN antibody used in Western Blot (WB). GTX101222

GTX101222 WB Image

LYN antibody detects LYN protein by Western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
C. 30 μg Raji whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (GTX101222) dilution: 1:1000

Anti-LYN antibody used in Immunoprecipitation (IP). GTX101222

GTX101222 IP Image

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of LYN protein by 4 μg LYN antibody (GTX101222)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (GTX101222) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-LYN antibody used in IHC (Paraffin sections) (IHC-P). GTX101222

GTX101222 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LYN(GTX101222) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Ben-Khemis M et al. Redox Biol 2023; 67 : 102898 TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes.
Li JP et al. Nat Commun 2014; 5 : 3618 The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck.
实验应用 : WB, IP
种属反应 : Mouse
Lai WB et al. Mol Neurobiol 2013; Ligand-Dependent Activation of EphA4 Signaling Regulates the Proteolysis of Amyloid Precursor Protein Through a Lyn-Mediated Pathway.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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