产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human LYN. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888566
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
LYN proto-oncogene, Src family tyrosine kinase , JTK8 , p53Lyn , p56Lyn
细胞定位
Cell membrane,Nucleus,Cytoplasm,Golgi apparatus,Membrane
功能与背景
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
数据库
研究领域
產品图片
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody detects LYN (phospho Tyr397) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The expression pattern is based on the publication:PMID: 25409824
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635694 IHC-P Image
LYN (phospho Tyr397) antibody [HL122] detects LYN (phospho Tyr397) protein in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.Sample: Paraffin-embedded Human Matched Pair Tissue Array - Brain (Alzheimer's Disease + Normal) (GTX49382).LYN (phospho Tyr397) stained by LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635694 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN (phospho Tyr397) antibody [HL122] (GTX635694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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