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Lamin A + C抗体 [GT1137]

Recombinant antibody KO/KD Orthogonal
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774
Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774
Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

目录号 GTX00774

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 GT1137

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥5000)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:50000 - 1:200000
ICC/IF 1:50 - 1:1000
IHC-P 1:50 - 1:1000
IP 1:50 - 1:200
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

74 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein of hmuan Lamin A/C

纯化方式

Purified by affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

细胞定位

Nucleus,nucleoplasm

功能与背景

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

数据库

研究领域

產品图片

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT1137] (GTX00774) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of various samples using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

Orthogonal

WB analysis of NIH-3T3 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00774 Lamin A + C antibody [GT1137].
Red: Primary antibody
Blue: DAPI
Detection system: confocol
Dlution : 1:100

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

WB analysis of HeLa whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Dilution :1:10000-1:2560000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774

GTX00774 IHC-P Image

IHC-P analysis of hmuan breast cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Immunoprecipitation (IP). GTX00774

GTX00774 IP Image

IP analysis of K562 whole cell lysate using GTX00774 Lamin A + C antibody [GT1137].
Total protein amount : 200μg
IP antibody : 3μg
Dilution : 1:20000

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137].
Pink: Primary antibody
Blue: DAPI
Dlution : 1:100-1:1600

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of L929 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
The image was taken by Confocal microscopy.
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00774

GTX00774 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX00774 Lamin A + C antibody [GT1137].
Green: Primary antibody
Blue: DAPI
Dlution : 1:200

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

Orthogonal

WB analysis of C6 extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

Orthogonal

WB analysis of HeLa extracts using GTX00774 Lamin A + C antibody [GT1137].
WCE : whole cell lysate
CE : cytoplasmic extracts
NE : nuclear extracts
Dilution : 1:10000
Loading : 25μg

Anti-Lamin A + C antibody [GT1137] used in IHC (Paraffin sections) (IHC-P). GTX00774

GTX00774 IHC-P Image

IHC-P analysis of hmuan esophageal cancer tissue section using GTX00774 Lamin A + C antibody [GT1137].
Dlution : 1:400 - 1:3200

Anti-Lamin A + C antibody [GT1137] used in Western Blot (WB). GTX00774

GTX00774 WB Image

KO/KD

WB analysis of Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts using GTX00774 Lamin A + C antibody [GT1137].
Dilution : 1:50000
Loading : 25μg

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5000
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