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Lamin A + C抗体 [mab636]

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813
Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813
Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

目录号 GTX80813

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 mab636

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-Fr

种属反应

 Human, Mouse, Bovine, Dog, Pig
产品包装
200 μl (¥5300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:100 - 1:2000
ICC/IF 1:100
IHC-Fr 1:100
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

74 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

Tissue culture supernatant

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Pig

免疫原

Porcine lamin preparation.

纯化方式

Unpurified

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

细胞定位

Nucleus,nucleoplasm

功能与背景

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

数据库

研究领域

產品图片

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of Human fibroblast cells using GTX80813 Lamin A + C antibody [mab636].
Fixation : methanol
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of U251 cells using GTX80813 Lamin A + C antibody [mab636]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813

GTX80813 WB Image

WB analysis of 10μg nuclear extract from fractionated mouse tissue lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:500

Anti-Lamin A + C antibody [mab636] used in Western Blot (WB). GTX80813

GTX80813 WB Image

WB analysis of various amounts of human fibroblast whole cell lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:2000

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of U2-OS cells (A) or HeLa cells (B) using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX80813 Lamin A + C antibody [mab636].
Red : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of A549 cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

Anti-Lamin A + C antibody [mab636] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80813

GTX80813 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80813 Lamin A + C antibody [mab636].

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产品包装 报价 (¥)
¥ 5300
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