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Lamin A + C抗体

KO/KD
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126
Anti-Lamin A + C antibody used in Immunoprecipitation (IP). GTX101126
Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101126

目录号 GTX101126

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Lamin A + C 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:66-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

74 kDa. ( Note )

阳性对照

C2C12 , NIH-3T3 , HeLa

预测种属反应

Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10617300

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

细胞定位

Nucleus

功能与背景

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

数据库

研究领域

產品图片

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126

GTX101126 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:1000.

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at membrane on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126

GTX101126 WB Image

Lamin A + C antibody detects Lamin A + C protein by Western blot analysis.
A. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin A + C antibody (GTX101126) dilution: 1:1000

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101126

GTX101126 WB Image

KO/KD

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101126) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101126

GTX101126 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Lamin A + C antibody (GTX101126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Immunoprecipitation (IP). GTX101126

GTX101126 IP Image

Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody (GTX101126).
Western blot analysis was performed using Lamin A + C antibody (GTX101126).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101126

GTX101126 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C (GTX101126) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

文献引用

Application Reference

Liang CJ et al. Cell Rep 2019; 28 (6) : 1511-1525.e5 SFRPs Are Biphasic Modulators of Wnt-Signaling-Elicited Cancer Stem Cell Properties beyond Extracellular Control.
实验应用 : WB
种属反应 : Mouse
Perrigue PM et al. Mol Cancer Res 2015; 13 (4) : 636-50 The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines.
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产品包装 报价 (¥)
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¥ 1700
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