Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HepG2 cytosol lysate/extract
C. 30 μg HepG2 nuclear lysate/extract
7.5% SDS-PAGE
Lamin B2 antibody [GT144] (GTX628803) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (GTX628803) diluted at 1:1000.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] (GTX628803) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] (GTX628803) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.