产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
Assay dependent |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4
细胞定位
Cytoplasm, cytoskeleton,Cell projection, dendrite
功能与背景
Enables microtubule binding activity. Involved in microtubule cytoskeleton organization; negative regulation of axon extension; and regulation of protein localization. Acts upstream of or within several processes, including establishment of cell polarity; microtubule bundle formation; and neuron projection development. Located in several cellular components, including axon; dendrite; and proximal neuron projection. Is expressed in several structures, including back skin; central nervous system; peripheral nervous system ganglion; retina; and trigeminal nerve. Orthologous to human MAP2 (microtubule associated protein 2). [provided by Alliance of Genome Resources, Apr 2022]
数据库
研究领域
產品图片
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GTX637253 ICC/IF Image
MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637253 IHC-P Image
MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637253 IHC-P Image
MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637253 IHC-P Image
MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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