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MCP1 / CCL2抗体 [2D8]

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Frozen sections) (IHC-Fr). GTX60582

目录号 GTX60582

文献引用 7

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 2D8

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

种属反应

 Human, Mouse, Rat, Monkey
产品包装
100 μg (¥5600)
查看所有 MCP1 / CCL2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

11 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Purified recombinant fragment of human CCL2 expressed in E. Coli.

纯化方式

Protein G Purified

偶联

Unconjugated

RRID

AB_2887982

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF

细胞定位

Secreted

功能与背景

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582

GTX60582 ELISA Image

ELISA analysis of antigen using GTX60582 MCP1 / CCL2 antibody [2D8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582

GTX60582 FACS Image

FACS analysis of A549 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Red : negative control

Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582

GTX60582 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of human CCL2 (AA: 1-99) recombinant protein using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582

GTX60582 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Frozen sections) (IHC-Fr). GTX60582

GTX60582 IHC-Fr Image

The data was published in the journal J Neuroinflammation in 2014. PMID: 25012628

文献引用

Application Reference

TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
Xu C et al. Oxid Med Cell Longev 2021; 2021 : 2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
实验应用 : WB
种属反应 : Mouse
Liao MF et al. Cells 2020; 9 (7) Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.
实验应用 : IHC-Fr
种属反应 : Rat
Guo SS et al. Cancer Lett 2019; 468 : 27-40 Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.
实验应用 : WB
种属反应 : Human
Shang J et al. Toxicol Sci 2018; (Epub) Recovery from a myocardial infarction is impaired in male C57bl/6N mice acutely exposed to the bisphenols and phthalates that escape from medical devices used in cardiac surgery.
实验应用 : WB
种属反应 : Mouse
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Application Reference
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
Xu C et al. Oxid Med Cell Longev 2021; 2021:2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
实验应用 : WB
种属反应 : Mouse
Liao MF et al. Cells 2020; 9 (7) Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.
实验应用 : IHC-Fr
种属反应 : Rat
Guo SS et al. Cancer Lett 2019; 468:27-40 Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.
实验应用 : WB
种属反应 : Human
Shang J et al. Toxicol Sci 2018; (Epub) Recovery from a myocardial infarction is impaired in male C57bl/6N mice acutely exposed to the bisphenols and phthalates that escape from medical devices used in cardiac surgery.
实验应用 : WB
种属反应 : Mouse
Wu H et al. Int J Mol Med 2018; (Epub) TIM-4 blockade of KCs combined with exogenous TGF-β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
实验应用 : WB
种属反应 : Mouse
McMillin M et al. J Neuroinflammation 2014; 11 (1):121 Neuronal CCL2 is upregulated during hepatic encephalopathy and contributes to microglia activation and neurological decline.
SDS
PBS.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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