产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX89355-PEP).
产品说明
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
预测种属反应
Rat, Bovine, Cat, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
MDM2 proto-oncogene , ACTFS , HDMX , hdm2
细胞定位
nucleoplasm , Cytoplasm , Nucleolus
功能与背景
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
数据库
研究领域
產品图片
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GTX89355 WB Image
WB analysis of HEK293 overexpressing MDM2 (mock transfection in first lane) using GTX89355 MDM2 antibody, Internal.
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GTX89355 WB Image
WB analysis of human liver lysate using GTX89355 MDM2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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文献引用
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产品评论
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