产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , 293T
预测种属反应
Mouse, Rat, Bovine, Dog, Chicken, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human MEK2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888214
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2
功能与背景
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541](/templates/default/js/lightbox/images/loading.gif "Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541")
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GTX630541 WB Image
Wild-type (WT) and MEK2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody [GT1162] (GTX630541) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX630541 WB Image
MEK2 antibody [GT1162] detects MEK2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
MEK2 antibody [GT1162] (GTX630541) dilution: 1:1000
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文献引用
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产品评论
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![Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541](/upload/website/prouct_img/small/GTX630541/GTX630541_41568_20180316_WB_KO_watermark_w_23061202_550.webp "Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541")
![Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541](/upload/website/prouct_img/small/GTX630541/GTX630541_41568_WB_w_23061202_172.webp "Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541")
![Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541](/upload/website/prouct_img/normal/GTX630541/GTX630541_41568_20180316_WB_KO_watermark_w_23061202_550.webp "Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541")
![Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541](/upload/website/prouct_img/normal/GTX630541/GTX630541_41568_WB_w_23061202_172.webp "Anti-MEK2 antibody [GT1162] used in Western Blot (WB). GTX630541")
