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MERS-CoV Nucleoprotein抗体

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868
Anti-MERS-CoV Nucleoprotein antibody used in ELISA (ELISA). GTX134868
Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

目录号 GTX134868

文献引用 1
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ELISA

种属反应

 Middle East respiratory syndrome coronavirus
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects MERS-CoV Nucleoprotein, but does not cross-react with SARS-CoV or SARS-CoV-2 nucleocapsid proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Middle East respiratory syndrome coronavirus

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of MERS-CoV Nucleoprotein (MERS-CoV (strain United Kingdom/H123990006/2012)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887364

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

研究领域

產品图片

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

GTX134868 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MERS-CoV Nucleoprotein antibody used in ELISA (ELISA). GTX134868

GTX134868 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant MERS-CoV Nucleocapsid protein (GTX135653-pro) (88.75-1.39 nM). Coated protein was probed with MERS-CoV Nucleoprotein antibody (GTX134868) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868

GTX134868 WB Image

Non-infected and infected Vero B4 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with MERS-CoV Nucleoprotein antibody (GTX134868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Weskamm LM et al. Cell Rep Med 2022; 3 (7) : 100685 Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine.
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产品评论

MERS-CoV Nucleoprotein antibody 目录号 GTX134868

Rating
( Average 3 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-MERS-CoV Nucleoprotein antibody used in Western Blot (WB). GTX134868
Date : Anonymous submitted on 21-Jan-2020
Score :
Application Tested : WB
Sample Species : MERS-CoV
Sample : Vero B4 cells
Amount used : 20 μg
Blocking : 5% milk in PBST, 20°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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