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MFF抗体 [HL1311]

Recombinant antibody Orthogonal
Anti-MFF antibody [HL1311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636729
Anti-MFF antibody [HL1311] used in Western Blot (WB). GTX636729
Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729
Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729

目录号 GTX636729

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1311

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

38 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910060

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mitochondrial fission factor , C2orf33 , EMPF2 , GL004

功能与背景

This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

数据库

研究领域

產品图片

Anti-MFF antibody [HL1311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636729

GTX636729 ICC/IF Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:4500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MFF antibody [HL1311] used in Western Blot (WB). GTX636729

GTX636729 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1311] (GTX636729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729

GTX636729 IHC-P Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MFF antibody [HL1311] used in IHC (Paraffin sections) (IHC-P). GTX636729

GTX636729 IHC-P Image

MFF antibody [HL1311] detects MFF protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MFF stained by MFF antibody [HL1311] (GTX636729) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品评论

MFF antibody [HL1311] 目录号 GTX636729

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-MFF antibody [HL1311] used in Western Blot (WB). GTX636729
Date : Anonymous submitted on 12-Nov-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse Brain tissue
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 16Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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