产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human MFF. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910061
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
mitochondrial fission factor , C2orf33 , EMPF2 , GL004
功能与背景
This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
数据库
研究领域
產品图片
![Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730](/templates/default/js/lightbox/images/loading.gif "Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730")
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GTX636730 WB Image
U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1312] (GTX636730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636730 ICC/IF Image
MFF antibody [HL1312] detects MFF protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MFF stained by MFF antibody [HL1312] (GTX636730) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX636730 ICC/IF Image
MFF antibody [HL1312] detects MFF protein at mitochondria by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: MFF stained by MFF antibody [HL1312] (GTX636730) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730](/upload/website/prouct_img/small/GTX636730/GTX636730_44620_20220318_WB_Fraction_w_23061202_867.webp "Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730")
![Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730](/upload/website/prouct_img/small/GTX636730/GTX636730_44620_20220422_ICC_IF_w_23061202_308.webp "Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730")
![Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730](/upload/website/prouct_img/small/GTX636730/GTX636730_T-44564_20220311_ICC_IF_w_23061202_877.webp "Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730")
![Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730](/upload/website/prouct_img/normal/GTX636730/GTX636730_44620_20220318_WB_Fraction_w_23061202_867.webp "Anti-MFF antibody [HL1312] used in Western Blot (WB). GTX636730")
![Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730](/upload/website/prouct_img/normal/GTX636730/GTX636730_44620_20220422_ICC_IF_w_23061202_308.webp "Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730")
![Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730](/upload/website/prouct_img/normal/GTX636730/GTX636730_T-44564_20220311_ICC_IF_w_23061202_877.webp "Anti-MFF antibody [HL1312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636730")
