产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
0.01-0.03μg/ml |
5μg/ml |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX89535-PEP).
预测种属反应
Mouse, Rat(>80% identity)
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
macrophage migration inhibitory factor , GIF , GLIF , MMIF
细胞定位
Secreted , Cytoplasm
功能与背景
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX89535 WB Image
WB analysis of Daudi (A) and Jurkat (B) lysate using GTX89535 MIF antibody, C-term. Dilution : 0.01μg/ml Loading : 35μg protein in RIPA buffer
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GTX89535 IHC-P Image
IHC-P analysis of human thymus using GTX89535 MIF antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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GTX89535 WB Image
WB analysis of human thymus lysate using GTX89535 MIF antibody, C-term. Dilution : 0.01μg/ml Loading : 35μg protein in RIPA buffer
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GTX89535 ELISA Image
ELISA analysis of MIF protein using GTX89535 MIF antibody, C-term. Capture : GTX101162 (5μg/ml) Detection : GTX89535 (1.5μg/ml)
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