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MIF抗体 [N1C3]

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162
Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162
Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162
Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162
Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162
Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

目录号 GTX101162

文献引用 4
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 MIF 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX101162 , Detection: GTX89535
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

12 kDa. ( Note )

阳性对照

Molt-4 , mouse brain

预测种属反应

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950920

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

macrophage migration inhibitory factor , GIF , GLIF , MMIF

功能与背景

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

GTX101162 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX101162 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162

GTX101162 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MIF antibody [N1C3] used in ELISA (ELISA). GTX101162

GTX101162 ELISA Image

ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL.

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MIF antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101162

GTX101162 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.

Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162

GTX101162 IHC-P Image

MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Murata R et al. Pharmaceutics 2022; 14 (3) Long-Acting Thioredoxin Ameliorates Doxorubicin-Induced Cardiomyopathy via Its Anti-Oxidative and Anti-Inflammatory Action.
实验应用 : WB
种属反应 : Mouse
Chen LL et al. Evid Based Complement Alternat Med 2021; 2021 : 5205759 Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice.
实验应用 : IHC-Fr
种属反应 : Mouse
Yulizal OK et al. J Adv Vet Anim Res 2020; 7 (2) : 209-217 The effect of snakehead fish extract supplementation to first-line eradication regimen on macrophage migration inhibitory factor (MIF) expression in rats induced by Helicobacter pylori infection.
实验应用 : IHC-P
种属反应 : Rat
Movila A et al. J Bone Miner Res 2016; (Epub) Macrophage Migration Inhibitory Factor (MIF) Supports Homing of Osteoclast Precursors to Peripheral Osteolytic Lesions.
实验应用 : IHC-P
种属反应 : Mouse
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产品评论

MIF antibody [N1C3] 目录号 GTX101162

Rating
( Average 4.5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-MIF antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101162
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
Anti-MIF antibody [N1C3] used in Western Blot (WB). GTX101162
Date : Anonymous submitted on 25-Jul-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 30μg
Blocking : milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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