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MITF抗体

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738
Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738
Anti-MITF antibody used in Western Blot (WB). GTX00738

目录号 GTX00738

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, ChIP assay

种属反应

Human, Mouse, Chicken, Xenopus
产品包装
100 μl (¥5600)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:3000
ICC/IF Assay dependent
IHC-P 1:300-1:1000
IHC-Fr 1:300-1:1000
ChIP assay 1:200
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

产品说明

This antibody recognizes MiTF-M, MiTF-A, MiTF-C, MiTF-H and other MiTF isoforms.

产品属性

形式

Liquid

存储溶液

Serum

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Human

免疫原

Recombinant full-size human Mitf (isoform M) protein with His tag

纯化方式

Unpurified

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

melanocyte inducing transcription factor , CMM8 , COMMAD , MI , WS2 , WS2A , bHLHe32

细胞定位

Nucleus

功能与背景

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of Mouse primary melanocytes (6 weeks) using GTX00738 MITF antibody.
Dilution : 1:500

Anti-MITF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00738

GTX00738 ICC/IF Image

ICC/IF analysis of mouse melanoma B16 cells using GTX00738 MITF antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization : 0.25 % Triton X-100

Anti-MITF antibody used in IHC (Frozen sections) (IHC-Fr). GTX00738

GTX00738 IHC-Fr Image

IHC-Fr analysis of chick embryo (stage 14, 8 μm) using GTX00738 MITF antibody.
At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Dilution : 1:300
Fixation : 4% PFA

Anti-MITF antibody used in Western Blot (WB). GTX00738

GTX00738 WB Image

WB analysis of various samples using GTX00738 MITF antibody.
Untreared : human melanoma cells
Control : human melanoma cells treated with control siRNA
Mitf : human melanoma cells treated with Mitf siRNA
Dilution : 1:1000

文献引用

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SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
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