产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910000
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
matrix metallopeptidase 2 , CLG4 , CLG4A , MMP-2 , MMP-II , MONA , TBE-1
细胞定位
Membrane,Nucleus,Mitochondrion
功能与背景
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
数据库
研究领域
產品图片
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/templates/default/js/lightbox/images/loading.gif "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
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GTX636524 WB Image
Non-transfected (–) and transfected (+) U87-MG whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636524 WB Image
Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636524 WB Image
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636524 IHC-P Image
MMP2 antibody [HL1207] detects MMP2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.MMP2 stained by MMP2 antibody [HL1207] (GTX636524) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/small/GTX636524/GTX636524_44522_20220128_WB_shRNA_watermark_w_23061202_657.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/small/GTX636524/GTX636524_44522_20220318_WB_M_skin_w_23061202_969.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/small/GTX636524/GTX636524_44522_20211210_WB_Fraction_w_23061202_297.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in IHC (Paraffin sections) (IHC-P). GTX636524](/upload/website/prouct_img/small/GTX636524/GTX636524_44522_20221227_IHC-P_multiple_M_22122821_107.webp "Anti-MMP2 antibody [HL1207] used in IHC (Paraffin sections) (IHC-P). GTX636524")
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/normal/GTX636524/GTX636524_44522_20220128_WB_shRNA_watermark_w_23061202_657.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/normal/GTX636524/GTX636524_44522_20220318_WB_M_skin_w_23061202_969.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524](/upload/website/prouct_img/normal/GTX636524/GTX636524_44522_20211210_WB_Fraction_w_23061202_297.webp "Anti-MMP2 antibody [HL1207] used in Western Blot (WB). GTX636524")
![Anti-MMP2 antibody [HL1207] used in IHC (Paraffin sections) (IHC-P). GTX636524](/upload/website/prouct_img/normal/GTX636524/GTX636524_44522_20221227_IHC-P_multiple_M_22122821_107.webp "Anti-MMP2 antibody [HL1207] used in IHC (Paraffin sections) (IHC-P). GTX636524")
