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MUC1抗体

Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459
Anti-MUC1 antibody used in Western Blot (WB). GTX100459
Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459
Anti-MUC1 antibody used in Immunoprecipitation (IP). GTX100459
Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459
Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459

目录号 GTX100459

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP

种属反应

Human
产品包装
100 μl (¥3590),
25 μl (¥1390)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

122 kDa. ( Note )

阳性对照

MCF-7

产品属性

形式

Liquid

存储溶液

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human MUC1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618561

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM

细胞定位

Apical cell membrane; Single-pass type I membrane protein , Isoform 5: Secreted , Isoform 7: Secreted , Isoform 9: Secreted , Mucin-1 subunit beta: Cell membrane , Cytoplasm , Nucleus

功能与背景

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459

GTX100459 IHC-P Image

MUC1 antibody detects MUC1 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
MUC1 stained by MUC1 antibody (GTX100459) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

Anti-MUC1 antibody used in Western Blot (WB). GTX100459

GTX100459 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC1 antibody (GTX100459) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 21927028

Anti-MUC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100459

GTX100459 IHC-P Image

MUC1 antibody detects MUC1 protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
MUC1 antibody (GTX100459) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC1 antibody used in Immunoprecipitation (IP). GTX100459

GTX100459 IP Image

Immunoprecipitation of MUC1 protein from HCT-116 whole cell extracts using 5 μg of MUC1 antibody (GTX100459).
Western blot analysis was performed using MUC1 antibody (GTX100459) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459

GTX100459 ICC/IF Image

MUC1 antibody detects MUC1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: MUC1 protein stained by MUC1 antibody (GTX100459) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MUC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100459

GTX100459 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUC1(GTX100459) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

文献引用

产品评论

MUC1 antibody 目录号 GTX100459

Rating ( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
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