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MUC2抗体 [C3], C-term

Orthogonal
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

目录号 GTX100664

文献引用 41
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FISH, IHC

种属反应

 Human, Mouse, Rat, Pig
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 MUC2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FISH Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

540 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950958

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mucin 2, oligomeric mucus/gel-forming , MLP , MUC-2 , SMUC

细胞定位

Secreted

功能与背景

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016]

数据库

研究领域

產品图片

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein in human intestine by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
MUC2 antibody [C3], C-term (GTX100664) diluted at 1:750.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100664

GTX100664 IHC-P Image

MUC2 antibody [C3], C-term detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (GTX100664) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32283838

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32283838

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32283838

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32283838

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32283838

Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664

GTX100664 ICC/IF Image

Orthogonal

The data was published in the journal Antioxidants (Basel) in 2020. PMID: 32150901

文献引用

Application Reference

Delaroque C et al. NPJ Biofilms Microbiomes 2024; 10 (1) : 1 Dietary emulsifier consumption accelerates type 1 diabetes development in NOD mice.
Singh AK et al. Cell Death Dis 2023; 14 (9) : 639 MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epithelium.
Yang WH et al. iScience 2023; 26 (10) : 107883 Innate mechanism of mucosal barrier erosion in the pathogenesis of acquired colitis.
Duan C et al. Gut Microbes 2023; 15 (1) : 2233149 Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting Akkermansia-related propanoate metabolism.
Han Y et al. Gut Microbes 2023; 15 (1) : 2221485 Akkermansia muciniphila inhibits nonalcoholic steatohepatitis by orchestrating TLR2-activated γδT17 cell and macrophage polarization.
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产品评论

MUC2 antibody [C3], C-term 目录号 GTX100664

Rating
( Average 4 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Anti-MUC2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100664
Date : Anonymous submitted on 01-Nov-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : LS174T cells
Blocking : 2% BSA in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 2% Triton-X100 in PBS
Primary Antibody : 1:100, 4°C, 16Hr
我要评论产品
Application Reference
Delaroque C et al. NPJ Biofilms Microbiomes 2024; 10 (1):1 Dietary emulsifier consumption accelerates type 1 diabetes development in NOD mice.
Singh AK et al. Cell Death Dis 2023; 14 (9):639 MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epithelium.
Yang WH et al. iScience 2023; 26 (10):107883 Innate mechanism of mucosal barrier erosion in the pathogenesis of acquired colitis.
Duan C et al. Gut Microbes 2023; 15 (1):2233149 Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting Akkermansia-related propanoate metabolism.
Canale V et al. Cell Mol Gastroenterol Hepatol 2023; 16 (1):39-62 PTPN2 Is a Critical Regulator of Ileal Paneth Cell Viability and Function in Mice.
Han Y et al. Gut Microbes 2023; 15 (1):2221485 Akkermansia muciniphila inhibits nonalcoholic steatohepatitis by orchestrating TLR2-activated γδT17 cell and macrophage polarization.
V Cortez et al. Mucosal Immunol 2023; S1933-0219 (23):00042-9 Indoleamine 2,3-dioxygenase 1 regulates cell permissivity to astrovirus infection
实验应用 : ICC/IF, IHC-P
种属反应 : Human, Mouse
Ilani T et al. EMBO J 2023; 42 (2):e111869 The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases.
实验应用 : WB
种属反应 : Mouse
Wang Z et al. Redox Biol 2023; 60:102618 FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response.
实验应用 : IHC-P
Xu Y et al. Cell Biosci 2023; 13 (1):7 Activation of goblet cell Piezo1 alleviates mucus barrier damage in mice exposed to WAS by inhibiting H3K9me3 modification.
实验应用 : IHC-P
种属反应 : Mouse
He Q et al. Life Sci Alliance 2023; 6 (5) Tannins amount determines whether tannase-containing bacteria are probiotic or pathogenic in IBD.
实验应用 : IHC-P
种属反应 : Mouse
Johnson NM et al. EMBO Rep 2022; 23 (11):e55209 Autophagic state prospectively identifies facultative stem cells in the intestinal epithelium.
Hayase E et al. Cell 2022; 185 (20):3705-3719.e14 Mucus-degrading Bacteroides link carbapenems to aggravated graft-versus-host disease.
Yao Y et al. Cell 2022; 185 (7):1172-1188.e28 Mucus sialylation determines intestinal host-commensal homeostasis.
实验应用 : IHC-P
种属反应 : Human
Lin SJ et al. Front Cell Infect Microbiol 2022; 12:1042815 Acute infection with Brachyspira hyodysenteriae affects mucin expression, glycosylation, and fecal MUC5AC.
实验应用 : IHC-P
种属反应 : Pig
Liu Y et al. Front Cell Infect Microbiol 2022; 12:1035386 Reprogrammed fecal and mucosa-associated intestinal microbiota and weakened mucus layer in intestinal goblet cell- specific Piezo1-deficient mice.
实验应用 : IHC-P
种属反应 : Mouse
Torp AM et al. Appl Environ Microbiol 2022; 88 (14):e0073422 Local Delivery of Streptomycin in Microcontainers Facilitates Colonization of Streptomycin-Resistant Escherichia coli in the Rat Colon.
实验应用 : FISH
种属反应 : Rat
Puri P et al. Cell Mol Gastroenterol Hepatol 2022; 14 (3):643-668.e1 Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia.
实验应用 : IHC-P
种属反应 : Mouse
Sharpen JDA et al. Nat Commun 2022; 13 (1):45 Transglutaminase 3 crosslinks the secreted gel-forming mucus component Mucin-2 and stabilizes the colonic mucus layer.
实验应用 : WB
种属反应 : Mouse
Hye-Youn Cho et al. Toxicol Pathol 2021; 49 (5):1077-1099 Role for Mucin-5AC in Upper and Lower Airway Pathogenesis in Mice.
实验应用 : IHC-P
种属反应 : Mouse
Feiling Song et al. Front Cell Dev Biol 2021; 9:745412 An Active Fraction of Trillium tschonoskii Promotes the Regeneration of Intestinal Epithelial Cells After Irradiation.
实验应用 : IHC-P
种属反应 : Mouse
Nishio J et al. Sci Rep 2021; 11 (1):20231 Identification and characterization of a novel Enterococcus bacteriophage with potential to ameliorate murine colitis.
实验应用 : IHC-P
种属反应 : Mouse
Gudi?o V et al. Nat Commun 2021; 12 (1):2335 RAC1B modulates intestinal tumourigenesis via modulation of WNT and EGFR signalling pathways.
Layunta E et al. Cell Rep 2021; 34 (7):108757 IL-22 promotes the formation of a MUC17 glycocalyx barrier in the postnatal small intestine during weaning.
Miyazaki T et al. Sci Rep 2021; 11 (1):492 Novel FXR agonist nelumal A suppresses colitis and inflammation-related colorectal carcinogenesis.
实验应用 : IHC-P
种属反应 : Mouse
Ma S et al. Biomolecules 2020; 10 (4) Exogenous NAD+ Stimulates MUC2 Expression in LS 174T Goblet Cells via the PLC-Delta/PTGES/PKC-Delta/ERK/CREB Signaling Pathway.
Wang S et al. Theranostics 2020; 10 (22):10171-10185 Me6TREN targets β-catenin signaling to stimulate intestinal stem cell regeneration after radiation.
实验应用 : IHC-P
种属反应 : Mouse
Li C et al. Res Vet Sci 2020; 133:75-84 Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine.
实验应用 : IHC-P
种属反应 : Mouse
van der Hee B et al. Front Cell Dev Biol 2020; 8:375 Congruence of Transcription Programs in Adult Stem Cell-Derived Jejunum Organoids and Original Tissue During Long-Term Culture.
实验应用 : IHC-P
Ma S et al. Sci Rep 2020; 10 (1):5523 Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model.
实验应用 : ICC/IF
种属反应 : Human
Yeom J et al. Antioxidants (Basel) 2020; 9 (3) Oxyresveratrol Induces Autophagy via the ER Stress Signaling Pathway, and Oxyresveratrol-Induced Autophagy Stimulates MUC2 Synthesis in Human Goblet Cells.
实验应用 : ICC/IF
种属反应 : Human
Song S et al. Biochem Biophys Res Commun 2020; 521 (4):1070-1076 Somatostatin stimulates colonic MUC2 expression through SSTR5-Notch-Hes1 signaling pathway.
实验应用 : ICC/IF
种属反应 : Mouse
Toubai T et al. Nat Microbiol 2019; 4 (5):800-812 Host NLRP6 exacerbates graft-versus-host disease independent of gut microbial composition.
Hwang D et al. Food Chem Toxicol 2018; 118:880-888 Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells.
实验应用 : ICC/IF
种属反应 : Human
Ozcan HEA et al. Adv Med Sci 2018; 63 (2):296-300 Expression profile and cellular localizations of mucin proteins, CK7, and cytoplasmic p27 in Barrett's esophagus and esophageal adenocarcinoma.
实验应用 : IHC-P
种属反应 : Human
Jinfang et al. Digestive and Liver Disease 2018; (Epub) Peroxisome Proliferator-activated Receptor Gamma Activation Promotes Intestinal Barrier Function by Improving Mucus and Tight Junctions in a Mouse Colitis Model.
实验应用 : IHC-P
种属反应 : Mouse
Sun C et al. Inflamm Bowel Dis 2017; 23 (8):1338-1347 Cideb Deficiency Aggravates Dextran Sulfate Sodium-induced Ulcerative Colitis in Mice by Exacerbating the Oxidative Burden in Colonic Mucosa.
实验应用 : IHC-P
种属反应 : Mouse
Hwang D et al. J Funct Foods 2017; 35:146-158 Oxyresveratrol-containing Ramulus mori ethanol extract attenuates acute colitis by suppressing inflammation and increasing mucin secretion.
实验应用 : ICC/IF
种属反应 : Human
Usui T et al. Stem Cells International 2016; 2016:Article ID 7053872 Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment.
实验应用 : ICC/IF
种属反应 : Human
Wang K et al. Immunity 2014; 41 (6):1052-63 Interleukin-17 receptor a signaling in transformed enterocytes promotes early colorectal tumorigenesis.
实验应用 : IHC-Fr
种属反应 : Mouse
Grivennikov SI et al. Nature 2012; 491 (7423):254-8 Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
实验应用 : IHC
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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欣博盛生物科技有限公司
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