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MYF5抗体

Anti-MYF5 antibody used in Western Blot (WB). GTX87110
Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110
Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110
Anti-MYF5 antibody used in Western Blot (WB). GTX87110

目录号 GTX87110

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Monkey
产品包装
100 μg (¥5300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

28 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

The antiserum was produced against synthesized peptide derived from human MYF5 (21-70).

纯化方式

Purified by antigen-affinity chromatography
From serum

偶联

Unconjugated

RRID

AB_10724548

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

别名

MYF5 , bHLHc2 , myogenic factor 5

细胞定位

Nucleus

数据库

研究领域

產品图片

Anti-MYF5 antibody used in Western Blot (WB). GTX87110

GTX87110 WB Image

WB analysis of COS7 cell lysates using GTX87110 MYF5 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX87110

GTX87110 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87110

GTX87110 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87110 MYF5 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MYF5 antibody used in Western Blot (WB). GTX87110

GTX87110 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28467493

文献引用

Application Reference

Migocka-Patrza?ek M et al. Int J Mol Sci 2022; 23 (15) Unique Features of River Lamprey (Lampetra fluviatilis) Myogenesis.
Hernandez-Ancheyta L et al. Int J Parasitol 2017; (Epub) Trichinella spiralis muscle larvae excretory-secretory products induce changes in cytoskeletal and myogenic transcription factors in primary myoblast cultures.
实验应用 : WB
种属反应 : Mouse
Willkomm L et al. PLoS One 2017; 12 (5) : e0176609 p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle.
实验应用 : ICC/IF
种属反应 : Mouse
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产品评论

MYF5 antibody 目录号 GTX87110

Rating
( Average 3 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-MYF5 antibody used in Western Blot (WB). GTX87110
Date : Anonymous submitted on 08-Jan-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : C2C12 cells
Amount used : 40μg
Blocking : 1% BSA,10%HS,0.075% Tween 20 in Tris-Cl pH7.4, 25°C, 1Hr
Primary Antibody : 1:250, 4°C, 16Hr
条带说明 : As MYF5 is a key transcription factor in myogenic specification and initial differentiation, WB was performed on C2C12 mouse myoblasts cultured in proliferation medium (PM) and then shifted to differentiation medium (DM) to allow myotubes formation.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5300
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