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MYH10抗体 [GT246]

Orthogonal Overexpression
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

目录号 GTX634160

文献引用 4
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT246

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 MYH10 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

229 kDa. ( Note )

阳性对照

HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain

预测种属反应

Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888411

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

myosin heavy chain 10 , NMMHC-IIB , NMMHCB

功能与背景

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

数据库

研究领域

產品图片

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000.

Anti-MYH10 antibody [GT246] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634160

GTX634160 ICC/IF Image

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MYH10 antibody [GT246] used in IHC (Paraffin sections) (IHC-P). GTX634160

GTX634160 IHC-P Image

MYH10 antibody [GT246] detects MYH10 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MYH10 antibody [GT246] (GTX634160) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MYH10 antibody [GT246] used in IHC (Frozen sections) (IHC-Fr). GTX634160

GTX634160 IHC-Fr Image

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (GTX634160) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160

GTX634160 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MYH10 antibody [GT246] (GTX634160) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
实验应用 : ICC/IF
种属反应 : Human
Yamada S et al. Bioeng Transl Med 2023; 8 (3) : e10509 Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.
Fang ZM et al. Cell Prolif 2023; 56 (4) : e13386 Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation.
实验应用 : WB
种属反应 : Human
He Y et al. Clin Epigenetics 2022; 14 (1) : 101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.
实验应用 : WB
种属反应 : Human
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产品评论

MYH10 antibody [GT246] 目录号 GTX634160

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-MYH10 antibody [GT246] used in Western Blot (WB). GTX634160
Date : Anonymous submitted on 19-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : A10 cells
Amount used : 60μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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