产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:1000 |
Assay dependent |
1:150 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full-length protein expressed in 293T cell transfected with human SILV expression vector
纯化方式
Purified by affinity chromatography
From tissue culture supernatant
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
premelanosome protein , D12S53E , ME20 , ME20-M , ME20M , P1 , P100 , PMEL17 , SI , SIL , SILV , gp100
细胞定位
Golgi apparatus
功能与背景
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
数据库
研究领域
產品图片
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GTX83640 IHC-P Image
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 WB Image
WB analysis of HEK293T cells transfected with Melanoma gp100 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83640 Melanoma gp100 antibody [8A4]. Loading : 5 ug per lane
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GTX83640 IHC-P Image
IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 WB Image
WB analysis of various cell lines using GTX83640 Melanoma gp100 antibody [8A4]. Loading : 35 ug per lane
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GTX83640 IHC-P Image
IHC-P analysis of human prostate tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 ICC/IF Image
ICC/IF analysis of A549 cells using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 ICC/IF Image
ICC/IF analysis of COS7 cells transiently transfected with Melanoma gp100 plasmid using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human pancreas carcinoma tissue using GTX83640 Melanoma gp100 antibody [8A4].
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GTX83640 IHC-P Image
IHC-P analysis of human liver tissue using GTX83640 Melanoma gp100 antibody [8A4].
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文献引用
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产品评论
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