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Mesothelin抗体 [MSVA-235M] HistoMAX™

HistoMAX
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373
Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

目录号 GTX04373

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 MSVA-235M

同种型

 IgG2b

实验应用

 IHC-P

种属反应

 Human
产品包装
500 μl (¥8900),
100 μl (¥4200)
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产品说明

摘要

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6, the immunostaining is markedly reduced.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

69 kDa. ( Note )

阳性对照

Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.

Negative Control

Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining.

产品属性

形式

Liquid

存储溶液

PBS, 0.05% BSA (Please contact us for PBS only format)

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein

纯化方式

Protein A/G purified

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mesothelin , MPF , SMRP

细胞定位

Golgi apparatus , Secreted

功能与背景

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

数据库

研究领域

產品图片

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human stomach tissue section using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Strong predominantly cytoplasmic mesothelin immunostaining in a stomach cancer with diffuse growth pattern.

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human adenoid cystic breast cancer tissue section using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Adenoid cystic breast cancer with strong membranous mesothelin immunostaining in a subset of cells.

Anti-Mesothelin antibody [MSVA-235M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04373

GTX04373 IHC-P Image

IHC-P analysis of human endometroid ovarian cancer tissue section using GTX04373 Mesothelin antibody [MSVA-235M] HistoMAX™.
Ovary Strong predominantly membranous mesothelin staining in an endometroid ovarian cancer.

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产品包装 报价 (¥)
¥ 8900
¥ 4200
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