产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:2000 |
| 1:50-1:100 |
| 1:50 |
| 2ug/500ul |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
fermitin family member 2 , KIND2 , MIG2 , PLEKHC1 , UNC112 , UNC112B , mig-2
细胞定位
Cytoplasm,Cytoplasm, cytoskeleton,Nucleus
功能与背景
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
数据库
研究领域
產品图片
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/templates/default/js/lightbox/images/loading.gif "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
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GTX84506 WB Image
WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84506 Mig-2 antibody [14A11].
Loading : 5 ug per lane
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GTX84506 ICC/IF Image
ICC/IF analysis of COS7 cells transiently transfected with Mig-2 plasmid using GTX84506 Mig-2 antibody [14A11].
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GTX84506 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX84506 Mig-2 antibody [14A11].
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GTX84506 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX84506 Mig-2 antibody [14A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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GTX84506 WB Image
WB analysis of various samples using GTX84506 Mig-2 antibody [14A11].
Loading : 10 ug per lane
Dilution : 1:200
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GTX84506 IP Image
IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84506 Mig-2 antibody [14A11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate
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GTX84506 WB Image
WB analysis of various cell lines using GTX84506 Mig-2 antibody [14A11].
Loading : 35 ug per lane
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GTX84506 WB Image
WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10μg per lane) using GTX84506 Mig-2 antibody [14A11].
Dilution : 1:500
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文献引用
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产品评论
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![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_4428_WB_w_23061420_497.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_1113_ICCIF_w_23061420_539.webp "Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_1114_ICCIF_w_23061420_614.webp "Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506")
![Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_2798_IHC-P_w_23061420_210.webp "Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_3538_WB_w_23061420_101.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_3370_IP_w_23061420_125.webp "Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_4429_WB_w_23061420_206.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/small/GTX84506/GTX84506_4427_20181228_WB_w_23061420_414.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_4428_WB_w_23061420_497.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_1113_ICCIF_w_23061420_539.webp "Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_1114_ICCIF_w_23061420_614.webp "Anti-Mig-2 antibody [14A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84506")
![Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_2798_IHC-P_w_23061420_210.webp "Anti-Mig-2 antibody [14A11] used in IHC (Paraffin sections) (IHC-P). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_3538_WB_w_23061420_101.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_3370_IP_w_23061420_125.webp "Anti-Mig-2 antibody [14A11] used in Immunoprecipitation (IP). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_4429_WB_w_23061420_206.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
![Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506](/upload/website/prouct_img/normal/GTX84506/GTX84506_4427_20181228_WB_w_23061420_414.webp "Anti-Mig-2 antibody [14A11] used in Western Blot (WB). GTX84506")
