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Mig-2抗体 [9E4]

KO/KD Overexpression
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in Immunoprecipitation (IP). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507
Anti-Mig-2 antibody [9E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84507
Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

目录号 GTX84507

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 9E4

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Rat, Dog, Monkey
产品包装
100 μl (¥7300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:2000
ICC/IF 1:50
IHC-P 1:150
IP 2ug/500ul
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

78 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

纯化方式

Purified by affinity chromatography

偶联

Unconjugated

RRID

AB_10727911

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

fermitin family member 2 , KIND2 , MIG2 , PLEKHC1 , UNC112 , UNC112B , mig-2

细胞定位

Cytoplasm,Cytoplasm, cytoskeleton,Nucleus

功能与背景

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

数据库

研究领域

產品图片

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

WB analysis of various cell lines using GTX84507 Mig-2 antibody [9E4].
Loading : 35 ug per lane

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

KO/KD

WB analysis of wild type and Mig-2 knock out HeLa cell lysate(10μg per lane) using GTX84507 Mig-2 antibody [9E4].
Dilution : 1:1000

Anti-Mig-2 antibody [9E4] used in Immunoprecipitation (IP). GTX84507

GTX84507 IP Image

IP analysis of DDDDK tagged Mig-2 overexpressed HEK293T lysate using GTX84507 Mig-2 antibody [9E4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Western Blot (WB). GTX84507

GTX84507 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Mig-2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84507 Mig-2 antibody [9E4].
Loading : 5 ug per lane

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human colon tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Mig-2 antibody [9E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84507

GTX84507 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84507 Mig-2 antibody [9E4].

Anti-Mig-2 antibody [9E4] used in IHC (Paraffin sections) (IHC-P). GTX84507

GTX84507 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84507 Mig-2 antibody [9E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

文献引用

Application Reference

Fanny Eysert et al. bioRxiv 2019; (Epub) Alzheimer’s genetic risk factor FERMT2 (Kindlin-2) is regulated by risk allele-binding of miR-4504 and leads to synaptic dysfunction in an APP-dependent manner.
实验应用 : WB
Sullivan SE et al. Hum Mol Genet 2018; (Epub) Candidate-based screening via gene modulation in human neurons and astrocytes implicates FERMT2 in Aβ and TAU proteostasis.
实验应用 : WB
种属反应 : Human
Chapuis J et al. Acta Neuropathol 2016; Genome-wide, high-content siRNA screening identifies the Alzheimer's genetic risk factor FERMT2 as a major modulator of APP metabolism.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 7300
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