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Monoamine Oxidase B抗体 [N2C3]

Orthogonal
Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in ELISA (ELISA). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in Western Blot (WB). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in Western Blot (WB). GTX105970
Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970

目录号 GTX105970

文献引用 5
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, ELISA, IHC, Sandwich ELISA

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Monoamine Oxidase B 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX105970 , Detection: GTX89368
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

阳性对照

Mouse liver

预测种属反应

Rat, Bovine, Dog, Guinea pig, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950856

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

monoamine oxidase B

细胞定位

Mitochondrion outer membrane

功能与背景

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970

GTX105970 IHC-P Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970

GTX105970 IHC-P Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Monoamine Oxidase B antibody [N2C3] used in ELISA (ELISA). GTX105970

GTX105970 ELISA Image

ELISA detection of MAOB using GTX105970 for capture at a concentration of 2.5 μg/mL and GTX89368 for detection at a concentration of 0.5 μg/mL.

Anti-Monoamine Oxidase B antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105970

GTX105970 ICC/IF Image

Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Monoamine Oxidase B antibody [N2C3] used in Western Blot (WB). GTX105970

GTX105970 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Monoamine Oxidase B antibody [N2C3] used in Western Blot (WB). GTX105970

GTX105970 WB Image

Orthogonal

HepG2 whole cell and mitochondria extracts (8.1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (GTX105970) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Monoamine Oxidase B antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105970

GTX105970 IHC-P Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020.PMID: 32316576

文献引用

Application Reference

Ping-Ho Chen et al. Cancers (Basel) 2021; 13 (13) Genetic and Proteinic Linkage of MAO and COMT with Oral Potentially Malignant Disorders and Cancers of the Oral Cavity and Pharynx.
实验应用 : WB
种属反应 : Human
Yang YC et al. Int J Mol Sci 2020; 21 (8) Monoamine Oxidase B Expression Correlates with a Poor Prognosis in Colorectal Cancer Patients and Is Significantly Associated with Epithelial-to-Mesenchymal Transition-Related Gene Signatures.
Liu Y et al. Mol Neurobiol 2019; (Epub) Attenuation of Ischemic Stroke-Caused Brain Injury by a Monoamine Oxidase Inhibitor Involves Improved Proteostasis and Reduced Neuroinflammation.
实验应用 : WB
种属反应 : Mouse
Seong Su Kang et al. bioRxiv 2019; (Epub) The Norepinephrine Metabolite DOPEGAL Confers Locus Coeruleus Tau Vulnerability and Propagation via Asparagine Endopeptidase.
实验应用 : WB
种属反应 : Human
Kang SS et al. EMBO J 2018; (Epub) α-Synuclein stimulation of monoamine oxidase-B and legumain protease mediates the pathology of Parkinson's disease.
实验应用 : WB
种属反应 : Mouse
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Monoamine Oxidase B antibody [N2C3] 目录号 GTX105970

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实验应用
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-Monoamine Oxidase B antibody [N2C3] used in Immunohistochemistry (IHC). GTX105970
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : IHC
Sample : Drosophila brain
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Human gene express in drosophila tissue.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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