Denatured rat IgG were separated by 12% SDS-PAGE, and the membrane was blotted with Rat IgG (Heavy chain) antibody [GT782] (HRP) (GTX628460-01) diluted at 1:1000.

Mouse anti-Rat heavy chain antibody
Comparison of western blot analysis with mouse anti-Rat heavy chain antibody (GTX628460-01 diluted at 1:5000) (1) and traditional secondary antibody (GTX224125-01 diluted at 1:5000) (2).
A. 2 μg denature rat IgG