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Mouse Interferon gamma 蛋白, His tag (active)

Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro
Mouse Interferon gamma protein, His tag (active). GTX00283-pro

目录号 GTX00283-pro

实验应用

Functional Assay

Speciess

Mouse
产品包装
10 μg (¥2900)

产品应用

应用说明

IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246. 7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246. 7 cells were incubated in DMEM with IFN-γ (2 ng/ml) for 24h, then cells  were  observed  by  inverted  microscope.

Observed MW

17 kDa.

Default 3

8

产品属性

形式

Lyophilized powder

存储溶液

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

保存剂

ProClin 300

存放说明

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; His23~Cys155 (NP_032363.1)

表达系统

E. coli

纯度

> 95%

内毒素

< 1 EU/μg

偶联

Unconjugated

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

别名

interferon gamma , IFN-g , Ifg

功能与背景

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

数据库

研究领域

產品图片

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Functional Assay Image

Activation effect of GTX00283-pro Mouse Interferon gamma protein (active).
(A) Unstimulated Raw 264.7 cells cultured in DMEM.
(B) Raw 264.7 cells cultured in DMEM, stimulated with IFN gamma.

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Functional Assay Image

ELISA detection of iNOS protein expression from RAW 246.7 cells stimulated by GTX00283-pro Mouse Interferon gamma protein (active) at 2 ng/ml for 24hrs.

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Image

SDS-PAGE analysis of GTX00283-pro Mouse Interferon gamma protein (active).

Mouse Interferon gamma protein, His tag (active). GTX00283-pro

GTX00283-pro Image

WB analysis of GTX00283-pro Mouse Interferon gamma protein (active).

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SDS
Proclin.pdf
产品包装 报价 (¥)
¥ 2900
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