Mouse Monoclonal Antibody IgG Subclass Strip Kit

Culture supernatants from various IgG-expressing (and one IgA-expressing) mouse hybridoma clones were characterized using the Mouse Monoclonal Antibody IgG Subclass Strip Kit (Cat. No. GTX543056) to illustrate sample readouts. The strip contains immobilized capture antibodies specific for IgG1, IgG2a, IgG2b, IgG3, and the kappa light chain. The control line (C) confirms valid assay performance.

Sample order (left to right): NC (Negative Control), IgG1k, IgG2ak, IgG2bk, IgG3k, IgAk, IgG1l
Capture lines (top to bottom): C (Control), IgG1, IgG2a, IgG2b, IgG3, kappa
Note: The IgAk sample is included as a negative control and produces only a kappa band signal, supporting the assay’s specificity for mouse IgG subclasses.