产品应用
预期分子量
Observed MW
16 kDa.
产品属性
形式
Lyophilized powder
存储溶液
Reconstitute in 20mM Tris, 150mM NaCl pH8.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl pH8.0, 0.05% sarcosyl, 5% trehalose.
保存剂
5% Proclin300
存放说明
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-tag, Ala279~Ser390 (NP_035707.1)
表达系统
E. coli
纯度
> 97%
内毒素
<1.0EU per 1μg (determined by the LAL method)
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
细胞定位
Secreted,Extracellular space,Extracellular matrix
功能与背景
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016]
数据库
研究领域
產品图片
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GTX04319-pro WB Image
WB analysis of GTX04319-pro Mouse TGF beta 1 protein, His tag (active) using Mouse TGFb1 antibody.
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GTX04319-pro Image
SDS-PAGE of GTX04319-pro Mouse TGF beta 1 protein, His tag (active).
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GTX04319-pro Neutralizing/Inhibition Image
Cell viability was assessed by CCK-8 assay after incubation with recombinant GTX04319-pro Mouse TGF beta 1 protein, His tag (active) for 48h.
ED50 : 7.1μg/mL
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GTX04319-pro Functional Assay Image
Cell proliferation effect of GTX04319-pro Mouse TGF beta 1 protein, His tag (active) 48h observed by inverted microscope.
(A) A549 cells cultured in DMEM, stimulated with 12.5μg/mL TGF-β1
(B) Unstimulated A549 cells cultured in DMEM
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