产品应用
应用说明
TNF-a (Tumor necrosis factor), being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1 ng/ml, 10 ng/ml) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope. Cell necrosis after incubation with TNF-a (10 ng/ml) for 72h.
Observed MW
24 kDa.
Default 3
8
产品属性
形式
Lyophilized powder
存储溶液
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
保存剂
ProClin 300
存放说明
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Gly57~Leu235 (NP_001265530.1)
表达系统
HEK293 cells
纯度
> 95%
内毒素
< 1 EU/μg
偶联
Unconjugated
注意事项
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
相关讯息
别名
tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f
功能与背景
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
数据库
研究领域
產品图片
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GTX00288-pro Functional Assay Image
Cell necrosis effect of GTX00288-pro Mouse TNF alpha protein (active).
(A) Unstimulated A549 cells cultured in DMEM for 72hrs.
(B) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF alpha for 72hrs.
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GTX00288-pro Functional Assay Image
ELISA detection of secreted IL-1 beta expression from A549 cells stimulated by GTX00288-pro Mouse TNF alpha protein (active) at 10 ng/ml for 8hrs.
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GTX00288-pro Image
WB analysis of GTX00288-pro Mouse TNF alpha protein (active).
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GTX00288-pro Image
SDS-PAGE analysis of GTX00288-pro Mouse TNF alpha protein (active).
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