Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. All Genetex's frozen tissue products, including frozen tissue arrays, frozen tissue sections, and frozen tissue panels, can be used for both immunohistochemistry and in situ hybridization. These products are ideal for rapid cellular localization of RNA and protein expression, especially for antibodies that don't recognize paraffin embedded tissue. At least one of the tissue slides from each lot was stained with H & E to ensure the quality. Frozen Tissue Section Panel contains 5 to 8 different tissues on one slide. The tissue panels are 5-10 μm in thickness, mounted on positively charged glass slides, and fixed by cold acetone. Note: Each individual tissue spot should be >90% core retention and total core lost from 1 slide should be no more than 5%.