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Myc tag抗体

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046
Anti-Myc tag antibody used in Western Blot (WB). GTX115046

目录号 GTX115046

文献引用 21

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IP

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Myc tag 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IP 1:10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

Transfected 293T

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11169757

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Myc tag antibody

研究领域

產品图片

Anti-Myc tag antibody used in Immunoprecipitation (IP). GTX115046

GTX115046 IP Image

Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody (GTX115046). (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by GTX115046 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody (GTX115046) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Myc tag antibody detects Myc tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Myc-tag tagged protein stained by Myc tag antibody (GTX115046) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Myc tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115046

GTX115046 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag(GTX115046) antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

The data was published in the journal J Virol in 2016. PMID: 27489281

Anti-Myc tag antibody used in Western Blot (WB). GTX115046

GTX115046 WB Image

The data was published in the journal Oncogene in 2017. PMID: 28436950

文献引用

Application Reference

Healy MD et al. Cell 2023; 186 (10) : 2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Han-Yu Wang et al. Cells 2021; 10 (2) Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.
实验应用 : WB, ICC/IF
Satoh Y et al. J Gen Virol 2021; 102 (10) M protein of subacute sclerosing panencephalitis virus, synergistically with the F protein, plays a crucial role in viral neuropathogenicity.
实验应用 : WB
Kuge H et al. J Lipid Res 2020; 61 (12) : 1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Liu YH et al. Biosci Rep 2020; 40 (8) Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy.
实验应用 : IP
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Application Reference
Healy MD et al. Cell 2023; 186 (10):2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Han-Yu Wang et al. Cells 2021; 10 (2) Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.
实验应用 : WB, ICC/IF
Satoh Y et al. J Gen Virol 2021; 102 (10) M protein of subacute sclerosing panencephalitis virus, synergistically with the F protein, plays a crucial role in viral neuropathogenicity.
实验应用 : WB
Kuge H et al. J Lipid Res 2020; 61 (12):1747-1763 PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.
Liu YH et al. Biosci Rep 2020; 40 (8) Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy.
实验应用 : IP
Lai HJ et al. J Neurol Sci 2020; 413:116810 Electrophysiological parameters that contribute to the pathogenesis of familial amyloid polyneuropathy caused by transthyretin mutations.
实验应用 : WB
Cao C et al. Front Microbiol 2019; 10:3040 BICP0 Negatively Regulates TRAF6-Mediated NF-庥B and Interferon Activation by Promoting K48-Linked Polyubiquitination of TRAF6.
实验应用 : WB
Te Velthuis AJW et al. Nat Microbiol 2018; 3 (11):1234-1242 Mini viral RNAs act as innate immune agonists during influenza virus infection.
Montes-Chinea NI et al. Neurol Genet 2018; 4 (6):e282 Identification of a new SYT2 variant validates an unusual distal motor neuropathy phenotype.
实验应用 : ICC/IF
Aartjan te Velthuis et al. bioRxiv 2018; (Epub) Mini viral RNAs act as innate immune agonists during influenza virus infection.
实验应用 : WB
von Morgen P et al. Oncogene 2017; (Epub) MRE11 stability is regulated by CK2-dependent interaction with R2TP complex.
实验应用 : WB
Shen YR et al. PLoS Genet 2017; 13 (3):e1006631 SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.
实验应用 : ICC/IF
Chang KH et al. J Formos Med Assoc 2016; Functional properties of LRRK2 mutations in Taiwanese Parkinson disease.
实验应用 : WB
Wong MT et al. J Virol 2016; Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulating Membranous Viral Replication Complex Formation.
实验应用 : WB
Yen CS et al. Sci Rep 2016; 6:25082 Lovastatin causes FaDu hypopharyngeal carcinoma cell death via AMPK-p63-survivin signaling cascade.
实验应用 : IP
Ding Y et al. Dev Biol 2016; (Epub) Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.
实验应用 : WB, IP
Kuo HY et al. Glycobiology 2016; 26 (2):155-65 Galectin-3 modulates the EGFR signalling-mediated regulation of Sox2 expression via c-Myc in lung cancer.
Lin YC et al. J Leukoc Biol 2015; Syk is involved in NLRP3 inflammasome-mediated caspase-1 activation through adaptor ASC phosphorylation and enhanced oligomerization.
Thomas S et al. Hum Mutat 2014; 35 (1):137-46 A homozygous PDE6D mutation in Joubert syndrome impairs targeting of farnesylated INPP5E protein to the primary cilium.
实验应用 : WB
Guo YC et al. Neurology 2014; 83 (17):1554-61 Spinocerebellar ataxia 35: novel mutations in TGM6 with clinical and genetic characterization.
实验应用 : WB
Yang JH et al. Nucleic Acids Res 2014; 42 (15):9908-24 Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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