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Myelin basic protein抗体

Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Anti-Myelin basic protein antibody used in Western Blot (WB). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in Western Blot (WB). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

目录号 GTX133108

文献引用 4
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IHC, IHC (Free Floating)

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Myelin basic protein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
IHC (Free Floating) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

27 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Myelin Basic Protein. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886829

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

myelin basic protein , C76307 , Hmbpr , R75289 , golli-mbp , jve , mld , shi

细胞定位

Isoform 13: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 12: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 11: Myelin membrane; Peripheral membrane protein; Cytoplasmic side , Isoform 10: Myelin membr

功能与背景

The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Myelin Basic Protein protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin Basic Protein antibody (GTX133108) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin Basic Protein antibody detects Myelin Basic Protein protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin Basic Protein antibody (GTX133108) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Myelin Basic Protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108

GTX133108 IHC-Fr Image

Myelin Basic Protein antibody detects Myelin Basic protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Myelin Basic protein stained by Myelin Basic Protein antibody (GTX133108) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Anti-Myelin basic protein antibody used in Western Blot (WB). GTX133108

GTX133108 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic protein antibody (GTX133108) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in Western Blot (WB). GTX133108

GTX133108 WB Image

Mouse tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody (GTX133108) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic Protein antibody detects Myelin basic Protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic Protein stained by Myelin basic Protein antibody (GTX133108) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Myelin basic protein antibody used in IHC (Paraffin sections) (IHC-P). GTX133108

GTX133108 IHC-P Image

Myelin basic protein antibody detects Myelin basic protein protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Myelin basic protein stained by Myelin basic protein antibody (GTX133108) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

SH Chen et al. Neurotherapeutics 2024; 21 (3) : e00336 Ultrahigh frequency transcutaneous electrical nerve stimulation for neuropathic pain alleviation and neuromodulation
实验应用 : IHC-Fr
Ruiz-Palacios M et al. Sci Total Environ 2020; 708 : 134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.
实验应用 : ICC/IF
Doppler K et al. J Neurol Neurosurg Psychiatry 2018; (Epub) Neurofascin-155 IgM autoantibodies in patients with inflammatory neuropathies.
实验应用 : IHC
种属反应 : Human
Oliveira CR et al. Dev Biol 2017; (Epub) Pseudotyped baculovirus is an effective gene expression tool for studying molecular function during axolotl limb regeneration.
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产品评论

Myelin basic protein antibody 目录号 GTX133108

Rating
( Average 5 based on 2 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
IHC (Free Floating)(IHC (Free Floating))
( Average 5 based on 1 users reviews)
Anti-Myelin basic protein antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX133108
Date : Anonymous submitted on 25-Feb-2020
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Brain tissue
Blocking : 1% BSA in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Myelin basic protein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133108
Date : Anonymous submitted on 23-Nov-2016
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain tissue
Blocking : 0.25% TX-100, 4% normal goat serum, 20°C, 2Hr
Fixation : Free floating sections, immunofluorescence
Permeabilization /Antigen retrieval : 0.25% TX-100
Primary Antibody : 1:500, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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