N-Cadherin抗体 [8C11]

IP analysis of SH-SY5Y cell lysates using GTX19348 N-Cadherin antibody [8C11].
GTX19348 was used as IP and IB antibody
IP reaction : 5μg antibody / 300μg lysate
WB antibody dilution : 1:1000

WB analysis of tissue lysate and whole cell extract (30 ug) of Mouse Brain (Lane 1) and MCF-7 (Lane 2) using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000

IHC-P analysis of human breast tissue using GTX19348 N-Cadherin antibody [8C11].
A : isotype control
B : Primary antibody
C : Primary antibody with magnified section
Antigen retrieval : HEIR with a buffer (pH 6.2)
Dilution : 2 μg/ml

WB analysis of 25 μg of various lysates using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000

ICC/IF analysis of SH-SY5Y cells using GTX19348 N-Cadherin antibody [8C11]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

WB analysis of 25 ug of Hela (Lane 1), HUVEC (Lane 2), and C2C12 cell lysates (Lane 3) using GTX19348 N-Cadherin antibody [8C11].
Dilution : 1:1000 (Hela) and 1:500 (HUVEC and C2C12)

IHC-P analysis of human heart tissue using GTX19348 N-Cadherin antibody [8C11].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20