应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
Cross-reactivity may occur with other RAS proteins
预测种属反应
Zebrafish, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis, Xenopus tropicalis(>80% identity)
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.41 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human N-Ras Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10619748
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
别名
NRAS proto-oncogene, GTPase , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6
细胞定位
Cell membrane; Lipid-anchor; Cytoplasmic side , Golgi apparatus membrane; Lipid-anchor
功能与背景
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]
数据库
研究领域